Main Article Content

Abstract

BACKGROUND


The invasion of microorganisms into the root canal system poses a significant challenge in endodontic infections, especially in primary teeth. Enterococcus faecalis is a common culprit in such infections, necessitating effective antimicrobial strategies. This study aims to assess various irrigating solutions' antimicrobial efficacy against E. faecalis.


Aim: The aim of this study was to evaluate the antimicrobial efficacy of different irrigating solutions against E. faecalis.


METHODS


Six experimental groups, including lemon extract, chlorine dioxide solution, apple cider vinegar, Echinacea angustifolia, sodium hypochlorite, and a control group (normal saline), were evaluated. The antimicrobial activity was assessed using the agar-well diffusion method, and MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal Concentration) were determined.


RESULTS


Lemon extract and apple cider vinegar exhibited higher zones of inhibition compared to sodium hypochlorite, chlorine dioxide, and Echinacea angustifolia. Statistical analysis confirmed significant differences in antimicrobial efficacy between various groups. Lemon extract and apple cider vinegar demonstrated the lowest MIC and MBC values, indicating potent antimicrobial activity against E. faecalis.


CONCLUSIONS


This study highlights the antimicrobial efficacy of lemon extract and apple cider vinegar against E. faecalis, suggesting their potential as alternative irrigating solutions in endodontic practice. Further research is warranted to explore their clinical applicability and long-term effects on treatment outcomes.

Keywords

Antimicrobial, Irrigating Solutions, Paediatric Endodontics.

Article Details

How to Cite
Roocha Jigish Shah, Anup Panda, & Mira Govindbhai Virda. (2024). Comparative Evaluation of Antimicrobial Effect of Different Irrigating Solution Against Enterococcus Faecalis - An In-Vitro Study. Journal of Evolution of Medical and Dental Sciences, 13(6), 180–185. https://doi.org/10.14260/jemds.v13i6.668

References

  1. Salas-López EK, Casas-Flores S, López-Lozano NE, et al. Analysis of bacterial communities of infected primary teeth in a Mexican population. Med Oral Patol Oral Cir Bucal 2020;25(5):e668-74.
  2. Cogulu D, Uzel A, Oncag O, et al. Detection of enterococcus faecalis in necrotic teeth root canals by culture and polymerase chain reaction methods. Eur J Dent 2007;1(4):216-21.
  3. Chaugule VB, Panse AM, Gawali PN. Adverse reaction of sodium hypochlorite during endodontic treatment of primary teeth. Int J Clin Pediatr Dent 2015;8(2):153-6.
  4. Bektaš N, Gazić B, Avdić M. Antibacterial activity of citrus juices and apple vinegar on Gram positive and Gram negative bacteria. International Journal of Innovative Science and Research Technology 2019;4(11):837-41.
  5. Brar R, Goel M, Arora KS, et al. The antibacterial effect of herbal alternatives, Triphala and Turmeric, on Enterococcus faecalis-an in vitro study. South African Dental Journal 2019;74(6):293-6.
  6. Gandhi H, Dave DS, Shah N, et al. Comparative evaluation of antimicrobial efficacy of herbal v/s synthetic irrigating solutions: an in vitro study. Int J Oral Care Res 2021;9(1):8-10.
  7. Prasad SD, Goda PC, Reddy KS, et al Evaluation of antimicrobial efficacy of neem and aloe vera leaf extracts in comparison with 3% sodium hypochlorite and 2% chlorhexidine against E. Faecalis and C. albicans. Journal of Dr. NTR University of Health Sciences 2016;5(2):104-10.
  8. Wiegand I, Hilpert K, Hancock RE. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nature Protocols 2008;3(2):163-75.
  9. Ayhan H, Sultan N, Cirak M, et al. Antimicrobial effects of various endodontic irrigants on selected microorganisms. Int Endod J 1999;32(2):99–102.
  10. Bystrom A, Sundqvist G. The antibacterial action of sodium hypochlorite and EDTA in 60 cases of endodontic therapy. Int Endod J 1985;18(1):35-40.
  11. Rôças IN, Siqueira JF, Santos KR. Association of Enterococcus faecalis with different forms of periradicular diseases. J Endod 2004;30(5):315-20.
  12. Peciuliene V, Reynaud AH, Balciuniene I, et al. Isolation of yeasts and enteric bacteria in root-filled teeth with chronic apical periodontitis. Int Endod J 2001;34(6):429–34.
  13. Orstavik D, Haapasalo M. Disinfection by endodontic irrigants and dressings of experimentally infected dentinal tubules. Endod Dent Traumatol 1990;6(4):142-9.
  14. Distel JW, Hatton JF, Gillespie MJ. Biofilm formation in medicated root canals. J Endod 2002;28(10):689–93.
  15. Moorer WR, Wesselink PR. Factors promoting the tissue dissolving capability of sodium hypochlorite. International Endodontic Journal 1982;15(4):187–96.
  16. Sebatni MA, Kumar AA. Smear layer removal efficacy of herbal extracts used as endodontic irrigants: an in vitro study. Endodontology 2017;29(1):35-8.
  17. Trombetta D, Castelli F, Sarpietro MG, et al. Mechanisms of antibacterial action of three monoterpenes. Antimicrob Agents Chemother 2005;49(6):2474–8.
  18. Omidbeygi M, Barzegar M, Hamidi Z, et al. Antifungal activity of thyme, summer savory and clove essential oils against Aspergillus flavus in liquid medium and tomato paste. Food Control 2007;18(12):1518-23.
  19. Mohanty S, Ramesh S, Muralidharan NP. Antimicrobial efficacy of apple cider vinegar against Enterococcus faecalis and Candida albicans: an in vitro study. J Adv Pharm Educ Res 2017;7(2):137-41.
  20. Dornelles-Morgental R, Guerreiro-Tanomaru JM, de Faria-Júnior NB, et al. Antibacterial efficacy of endodontic irrigating solutions and their combinations in root canals contaminated with Enterococcus faecalis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2011;112(3):396-400.
  21. Soorya MS, Jayalakshmi KB, Prasannalatha N, et al. Comparative evaluation of antimicrobial effect of ginger, apple cider vinegar and fruit vinegar–an in vitro UV spectrophotometric study. International Journal of Science and Research 2019;8(8):2289-93.
  22. Showkat I, Chaudhary S, Sinha A, et al. Evaluation the antibacterial efficacy of chitosan, chlorohexdine,apple cider vinegar and sodium hypochorite on E.fecalis biofilm in primary teeth :an invitro study. E JPMR 2020;7(5):667-73.
  23. Kishen, A. Advanced therapeutic options for endodontic biofilms. Endodontic Topics 2009;22(1):99-123.
  24. Giardino L, Ambu E, Becce C, et al. Surface tension comparison of four common root canal irrigants and two new irrigants containing antibiotic. Journal of Endodontics 2006;32(11):1091-3.
  25. Herczegh A, Ghidan A, Friedreich D, et al. Effectiveness of a high purity chlorine dioxide solution in eliminating intracanal Enterococcus faecalis biofilm. Acta Microbiol Immunol Hung 2013;60(1):63-75.
  26. Aberna RA, Mohan G, Saranya S. Enterococcicidal activity of chlorine dioxide and lemon extract endorse them as contemporary root canal irrigants. Archives of Medicine and Health Sciences 2019;7(2):167-71.
  27. Sharma M, Vohra S, Arnason JT, et al. Echinacea extracts contain significant and selective activities against human pathogenic bacteria. Pharmaceutical Biology 2008;46(1):111-16.